《植物生理学报》 2014, 50(4): 461-470

番茄NAC转录因子家族的鉴定及生物信息学分析

陈秀玲¹, 王傲雪^1,2,*, 张珍珠^1,2, 李景富^1,*

东北农业大学¹园艺学院, ²生命科学学院, 哈尔滨 150030

通信作者：王傲雪；E-mail: axwang@neau.edu.cn, lijf_2005@126.com；Tel: 0451-55191671, 0451-55191671

摘　要：

NAC转录因子家族是植物特有的一类转录因子, 在植物的生长发育、器官建成及逆境胁迫和激素信号应答中均发挥重要作用。本研究在基因组范围内, 利用生物信息学方法对番茄的NAC转录因子家族成员、分布及结构和功能等进行分析。预测结果显示番茄NAC家族包含102个蛋白质, 分为12亚族, 其中茄属特有的TNAC亚族中成员最多, 具有25个, 其他NAC转录因子与拟南芥NAC家族具有相似分类。保守基序分析, 在番茄NAC结构域中包含7个保守的NAM基序, 主要分布在序列的N端, 表明这些基序的存在对NAC蛋白质功能的执行是必需的。理化性质和结构分析表明, 番茄NAC蛋白质绝大多数是亲水蛋白质, 主要以无规则卷曲构成, 而α-螺旋、β-折叠和β-转角则散布于整个蛋白质中, 在各亚族中没有规律。

关键词：番茄; NAC转录因子; 生物信息学

收稿：2013-12-17   修定：2014-03-05

资助：国家自然基金项目(31301780)、黑龙江省教育厅海外学人项目(1252HQ011)、黑龙江省博士后基金(LBH-Z12044)和东北农业大学“寒地蔬菜生物学重点实验室”开放基金(CVB2012-05)。

Genome-Wide Identification and Bioinformatics Analysis of NAC Gene Family in Tomato

CHEN Xiu-Ling¹, WANG Ao-Xue^1,2,*, ZHANG Zhen-Zhu^1,2, LI Jing-Fu^1,*

¹School of Horticulture, ²School of Life Science, Northeast Agricultural University, Harbin 150030, China

Corresponding author: WANG Ao-Xue; E-mail: axwang@neau.edu.cn, lijf_2005@126.com; Tel: 0451-55191671, 0451-55191671

Abstract:

NAC proteins belong to one of the largest plant-specific transcription factor families and play diverse roles in plant development processes, organ formation, response to biotic or abiotic cues, and hormone signalling. NAC proteins of tomato were genome-wide identified, the distribution on the chromosome, the structure and function of protein were analyzed. 102 predicted proteins constitute the tomato NAC transcrition factor family, which can be clustered into 12 groups. 25 of them belong to TNAC subfamiliy (a novel NAC subfamily which was first identified in tobacco), and other members have the samilar classification with Arabidopsis thaliana. Conservative motif analysis indicates that there are seven conserve NAM motif in N terminal. The physical and chemical properties and structure analysis showed that most of the tomato NAC proteins are hydrophilic proteins, the secondary structure of 102 predicted NAC proteins was mainly rendom coil, and alpha helix, beta sheet and beta turn are scattered to the whole protein sequence, there is no rule in each subfamily.

Key words: tomato; NAC transcription factor; bioinformatics